UBC Dentistry
Centre for High-Throughput Phenogenomics
ABOUT THE CENTRE
The Centre for High-Throughput Phenogenomics integrates imaging and molecular information across the spectrum from the molecular level (DNA, RNA and protein) through the microscopic (electron-, laser-, and light-microscopic) to the macroscopic level (Computed Tomography and morphological scanning) and allows quantitative analysis of form.
The broad goal of this centre is to answer questions such as "what molecular events make a face?" and "how can we help patients who have craniofacial (dental, bone, soft tissue) problems, whether these are inherited or acquired?"
Sample PREPARATION MODALITIES:
- Rapid freezing and cryotome for light and confocal microscopy and for laser-capture microdissection analysis
- Critical Point Drier, Fixation, embedding and microtomy (paraffin and plastic) for light microscopy, electron- and immunoelectron- microscopy
- Ultramicrotomy for transmission electron microscopy
- Sputter coating for scanning electron microscopy
- Tunable power-controlled microwave antigen-retrieval system, for immunohistochemistry
IMAGING AND ANALYSIS
- Zeiss-PALM Laser Microdissection and Capture Platform- laser microdissection and capture of tissue samples for RNA, DNA, and Protein analysis.
- Zeiss Axioplan Fluorescence Microscopes with digital image capture and analysis; Leica fluorescence microscope with digital image capture and Openlab Image Analysis.
- Nikon Confocal Microscope with digital image capture and analysis system.
- Cambridge Stereoscan 260 Digital Scanning Electron Microscope with Backscatter analysis.
- Phillips EM300 Transmission Electron Microscope.
