Dr. Richman’s Research
Embryology; Gene Expression and Function; Evolution
The early embryonic faces of humans, mice and reptiles are so similar that it is difficult to tell them apart. Yet through a series of steps, cells are patterned to form the intricate skeleton of the upper and lower jaws. My lab works on the molecular changes in early embryos that gradually build up the hard tissues of the face. The work follows three major themes, the establishment of jaw identity or jaw pattern, the basis for orofacial clefting and the evolution of jaws and teeth.
It is fascinating to study how individual parts of the embryonic face are specified to form either a nose, mandible, maxilla.. Past work from my lab has shown that by tweaking the levels of different molecules in the face, a striking change occurs, the maxilla is transformed into centre of the face (Lee et al., 2001). The chicken embryo is ideal for these experiments since the environment can be locally changed in many ways either by local release of chemicals or by surgical manipulation. We are presently extending these studies, by studying certain genes that were identified as being changed following the implantation of face transformation signals into early embryos.
The second focus area is cleft lip. The human form of cleft lip with or without cleft palate (CL/P) is a common birth defect and is most often not associated with a syndrome. It is thought that there is an interaction between the individual’s genes and the environment to produce the CL/P. Our experiments have identified important roles for certain growth factors in lip fusion and these have all subsequently been identified as contributing genes in human clefting.
The third focus area is evolution of the jaws and teeth. Here the embryos of snakes, lizards and turtles are being studied. Since living reptiles are most closely related to dinosaurs, our work is giving us insights into the development of now extinct animals. Recently we have identified dental epithelial stem cells in geckos that could underlie the ability of this animal to replace its teeth continuously. A detailed analysis of molecular signaling in python teeth corroborates and extends the work on the gecko. Ours are the first experiments to study the process of tooth replacement in animals that replace their teeth throughout life. Thus not only is our work telling us how during evolution, humans lost the ability to continually replace teeth, We also are gaining valuable insights into how tooth replacement in humans occurs.
- Life Sciences Institute Cell and Development Biology Research Group
- Cell tracking animation 1
- Cell tracking animation 2 (high power)
- Iso surface animation
- Limb bud transfection animation
- Buchtová M, Handrigan G, Tucker A, Lozanoff S, Town L, Fu KK, Diewert VM, Wicking C, Richman JM (2008). Initiation and patterning of the snake dentition is dependent on Sonic Hedgehog signalling. Dev Biol 319:132-145.
- Szabo-Rogers HL, Geetha-Loganathan P, Nimmagadda S, Richman JM (2008). FGF signals from the nasal slit are necessary for normal facial morphogenesis. Dev Biol 318:289-302.
- Szabo-Rogers HL, Geetha-Loganathan P, Whiting C, Nimmagadda S, Fu K, Richman JM (2009). Novel skeletogenic patterning roles for the olfactory pit. Development 136:219-229.
- Geetha-Loganathan P, Nimmagadda S, Antoni L, Fu K, Whiting CJ, Francis-West P, Richman JM (2009). Expression of WNT signalling pathway genes during chicken craniofacial development. Dev Dynam 238:1150-1165.
- Buchtová M, Nimmagadda S, Kuo WP, Benson SL, Geetha-Loganathan P, Logan C, Au-Yeung T, Chiang E, Fu K, Richman JM (2010). Whole genome microarray analysis of chicken embryo facial prominences. Dev Dynam 239:574-591.
- Handrigan GR, Richman JM (2010). Autocrine and paracrine Shh signaling is necessary for tooth morphogenesis, but not tooth replacement in snakes and lizards (Squamata). Dev Biol 337:171-186.
- Higashihori N, Buchtová M, Richman JM (2010). The function and regulation of TBX22 in avian frontonasal morphogenesis. Dev Dynam 239:458-473.
- Handrigan GR, Leung KJ, Richman JM (2010). Identification of putative dental epithelial stem cells in a lizard with life-long tooth replacement. Development 137:3545-3549.
- Handrigan GR, Richman JM (2010). A network of Wnt, hedgehog and BMP signaling pathways regulates tooth replacement in snakes. Dev Biol 348:130-141.
- Geetha-Loganathan P, Nimmagadda S, Hafez I, Fu K, Cullis PR, Richman JM (2011). Development of high-concentration lipoplexes for in vivo gene function studies in vertebrate embryos. Dev Dyn 240:2108-2119.
- Handrigan GR, Richman JM (2011). Unicuspid and bicuspid tooth crown formation in squamates. J Exp Zool B Mol Dev Evol DOI: 10.1002/jezmde.21438.